Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage
نویسندگان
چکیده
منابع مشابه
Comparison of the DNA Fragmentation and the Sperm Parameters after Processing by the Density Gradient and the Swim up Methods.
INTRODUCTION The swim up and the density-gradient centrifugation are the two main techniques which are used to separate the viable motile sperm fraction in the assisted reproductive technology. However, there are several published studies about these methods, but there is no sufficient evidence for recommending the superiority of one of them. This study was designed to study the efficiency of t...
متن کاملSperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation
Objective The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy, and abnormal chromatin structure. Methods Semen samples were obtained from 18 healthy male partners who attended infertility clinics for infertility investigations and were processed with swim-up and D...
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The Artificial Insemination (AI) is the first option treatment for infertile couples with cervical factor subfertility, mild-moderate male subfertility and unexplained infertility. With the exception of cases in which the use of in vitro fertilization (IVF or ICSI) is strictly due as a consequence of a severe male or female factor, the artificial insemination must be part of a gradual approach ...
متن کاملComparison of two sperm processing techniques for low complexity assisted fertilization: sperm washing followed by swim-up and discontinuous density gradient centrifugation
OBJECTIVE This study aimed to assess and compare sperm motility, concentration, and morphology recovery rates, before and after processing through sperm washing followed by swim-up or discontinuous density gradient centrifugation in normospermic individuals. METHODS Fifty-eight semen samples were used in double intrauterine insemination procedures; 17 samples (group 1) were prepared with sper...
متن کاملBull Fertility and Its Relation with Density Gradient Selected Sperm
Background sperm selection is generally used to describe methods for separation of spermatozoa for in-vitro-assisted reproduction. Few studies reported relation in vivo fertility and semen parameters after selection of fertile spermatozoa; hence, our research attempt to assess different semen parameters after post-thaw (PT) or after selecting sperm sample (SSp), using density gradient separatio...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2012
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-012-9742-x